Protein-Ligand Interactions: Methods and Applications by Mark A. Williams (Engli

Description: Protein-Ligand Interactions by Mark A. Williams, Tina Daviter In its revised 2nd Edition, this Methods in Molecular Biology(TM) book provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. Offers materials lists, reproducible protocols, notes and more. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description Proteins are the cells workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology. Back Cover Proteins are the cells workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions - whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology. Table of Contents Protein-Ligand Interactions: Fundamentals.- Protein Sample Characterization.- Measurement of Protein-Ligand Complex Formation.- Isothermal Titration Calorimetry for Studying Protein-Ligand Interactions.- Rapid Mixing Kinetic Techniques.- Protein-Ligand Interactions Using SPR Systems.- Fluorescence Techniques in Analysis of Protein-Ligand Interactions.- Circular and Linear Dichroism Spectroscopy for the Study of Protein-Ligand Interactions.- Analyzing Protein-Ligand Interactions by Dynamic NMR Spectroscopy.- Studying Metal Ion - Protein Interactions: Electronic Absorption, Circular Dichroism and Electron Paramagnetic Resonance.- Monitoring Protein-Ligand Interactions by Time-resolved FTIR Difference Spectroscopy.- Biophysical Methods in Drug Discovery from Small Molecule to Pharmaceutical.- Biophysical Screening for the Discovery of Small-Molecule Ligands.- Screening Protein-Small Molecule Interactions by NMR.- Model Membrane Systems.- Quantitative Fluorescence Co-localization to Study Protein-Receptor Complexes.- Studying Protein-Ligand Interactions Using X-Ray Crystallography.- Molecular Fields in Ligand Discovery.- Structure-Based Virtual Screening for Novel Ligands. Feature Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Details ISBN1627033971 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2013 Edition 2nd ISBN-10 1627033971 ISBN-13 9781627033978 Format Hardcover Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Tina Daviter DEWEY 615.19 Subtitle Methods and Applications Short Title PROTEIN-LIGAND INTERACTIONS 20 Language English Media Book Series Number 1008 Publication Date 2013-06-01 Pages 530 DOI 10.1007/978-1-62703-398-5 AU Release Date 2013-06-01 NZ Release Date 2013-06-01 US Release Date 2013-06-01 UK Release Date 2013-06-01 Author Tina Daviter Edition Description 2nd ed. 2013 Alternative 9781493958733 Audience Professional & Vocational Illustrations 45 Illustrations, color; 101 Illustrations, black and white; X, 530 p. 146 illus., 45 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96368997;

Price: 392.54 AUD

Location: Melbourne

End Time: 2025-01-07T09:14:33.000Z

Shipping Cost: 18.39 AUD